Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb
货号：
13129S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 13129S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb detects endogenous levels of FoxO3a only when phosphorylated at Ser253. This antibody may cross-react with FoxO1 when overexpressed and phosphorylated at Ser251 or FoxO4 when overexpressed and phosphorylated at Ser197. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).FoxO3a is phosphorylated by Akt at several sites, including Thr32, Ser253 and Ser315. FoxO3a associates with 14-3-3 proteins following Akt phosphorylation and is retained in the cytoplasm. In the absence of survival factors, FoxO3a is dephosphorylated, translocates to the nucleus, and triggers cell death by a Fas ligand-dependent mechanism (7). |
| 存放说明 | -20C |
| 计算分子量 | 97 |
参考图片Western blot analysis of extracts from 293T cells, untreated (-) or treated with LY294002 #9901 (50 μM, overnight; +) and Wortmannin #9951 (1 μM, overnight; +), using Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb (upper) or FoxO3a (D19A7) Rabbit mAb #12829 (lower). 未处理或经过LY294002 #9901 (50 μM, overnight; +)和Wortmannin #9951 (1 μM, overnight; +)处理的293T细胞提取物，使用Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb兔单抗 (上)或 FoxO3a (D19A7) Rabbit mAb兔单抗 #12829 (下)进行western blot分析。 |
