Phospho-GEF-H1 (Ser886) (E1L6D) Rabbit mAb
货号：
14143S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 14143S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). Phosphorylation of GEF-H1 at Ser886 (Ser885 in mouse), a site located in the 14-3-3 binding motif, has been implicated in recruitment of 14-3-3 and GEF-H1 to microtubules (3), and in the regulation of RhoA activity in response to mitotic kinases during cytokinesis (4). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
参考图片Western blot analysis of extracts from HT-29 and 293T cells using Phospho-GEF-H1 (Ser886) (E1L6D) Rabbit mAb in the presence of a nonphosphorylated synthetic peptide or a synthetic peptide phosphorylated at Ser886. |
Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Phospho-GEF-H1 (Ser886) (E1L6D) Rabbit mAb (upper) and GEF-H1 (55B6) Rabbit mAb #4076 (lower). |
