Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
货号：
11873S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 11873S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb recognizes endogenous levels of GIT2 protein only when phosphorylated at Tyr392. This antibody may cross-react weakly with other tyrosine-phosphorylated proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF Pix, and the focal adhesion protein paxillin (reviewed in 1). GIT1 and GIT2 share many of the same properties, but with at least ten distinct, tissue-specific splice variants. GIT2 has been shown to play an important role in inhibiting focal adhesion turnover and membrane protrusion (2,3). Focal adhesion localization and paxillin binding of GIT2 is regulated through phosphorylation at one or more tyrosine sites (Tyr286, Tyr392, Tyr592) by FAK and/or Src (4,5,reviewed in 6). Once at the focal adhesion, GIT2 is thought to play a key role in cell polarity and migration, making it a protein of interest in the investigation of oncogenic signaling pathways (3,5,7). |
| 存放说明 | -20C |
| 计算分子量 | 85 |
参考图片Western blot analysis of extracts from U-2 OS cells, untreated (-) or CIP and λ phosphatase-treated (+), using Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb (upper) or GIT2 (D11B8) Rabbit mAb #8072 (lower).未处理（-）或经过CIP和λ phosphatase-treated (+)处理里的U-2 OS细胞，使用Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb (上) 或GIT2 (D11B8) Rabbit mAb #8072 (下)进行western blot分析。 |
