| 货号 | 11817S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb recognizes endogenous levels of GRB10 protein only when phosphorylated at Ser476. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Recently GRB10 was shown to be a direct substrate of mTORC1 (5). It is phosphorylated by mTORC1 at Ser150, Ser428, and Ser476 upon insulin stimulation (5).The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from serum starved NIH/3T3 cells, untreated or treated with insulin (100 nM, 5 min), hIGF-1 #8917 (100 ng/ml, 5 min), or λ phosphatase, using Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 血清饥饿后的NIH/3T3不处理或经过胰岛素(100 nM, 5 min), hIGF-1 #8917 (100 ng/ml, 5 min), 或 λ phosphatase处理后获得细胞提取物，使用Phospho-GRB10 (Ser476) (D4E6)Rabbit mAb兔单抗（上）或β-Actin (D6A8) Rabbit mAb 兔单抗#8457(下)进行western blot分析。 | |
Immunoprecipitation of phospho-GRB10 (Ser476) from extracts of serum starved NIH/3T3 cells treated with insulin (100 nM, 5 min), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb. 血清饥饿后的NIH/3T3细胞经过胰岛素(100 nM, 5 min)处理获得提取物，使用Rabbit mAb IgG XP® Isotype Control #3900 (lane 2)或Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb 兔单抗(lane 3)对phospho-GRB10 (Ser476)进行免疫沉淀。Lane1是10%上样量。使用phospho-GRB10 (Ser476) (D4E6)mAb兔单抗进行western blot分析。 |
