| 货号 | 8452S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-GSK-3α (Ser21) (D1G2) Rabbit mAb recognizes endogenous levels of GSK-3α protein only when phosphorylated at Ser21. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of extracts from NIH/3T3 cells treated with PDGF, or COS-7 cells, either untreated or trested with λ phosphatase, using Phospho-GSK-3α (Ser21) (D1G2) Rabbit mAb (upper) and GSK-3α (D80E6) Rabbit mAb #4337 (lower). Western blot 分析NIH/3T3细胞提取物， PDGF处理组和 COS-7细胞，未处理组或λ 磷酸酶处理组, 所用抗体为 Phospho-GSK-3α (Ser21) (D1G2) Rabbit mAb 兔单抗(上) 和GSK-3α (D80E6) Rabbit mAb兔单抗 #4337 (下）。 |
