| 货号 | 8566S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb recognizes endogenous levels of GSK-3α and GSK-3β proteins only when phosphorylated at Ser21 or Ser9, respectively. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 46 GSK-3beta, 51 GSK-3alpha |
Western blot analysis of extracts from GSK-3α (-/-) and GSK-3β (-/-) mouse embryonic fibroblasts (MEF), untreated or insulin-treated (100 ng/ml, 20 min) , using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). Western blot 分析GSK-3α (-/-) 或 GSK-3β (-/-) (泳道 5,6) 小鼠胚胎成纤维细胞 (MEFs), 未处理组或 胰岛素处理组(100 ng/ml, 20 min), 所用抗体为Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb兔单抗 (上) 和GSK-3α/β (D75D3) XP ® Rabbit mAb 兔单抗#5676 (下). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) 细胞是加拿大多伦多大学的Dr. Jim Woodgett友情赠送). | |
Western blot analysis of extracts from COS-7 cells, λ phosphatase- or PDGF-treated (100 μg/ml, 15 min), and PC-3 cells, untreated or λ phosphatase-treated, using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). Western blot 分析COS-7 细胞提取物， λ 磷酸酶或PDGF处理组(100 μg/ml, 15分钟), 和 PC-3 细胞，未处理组或λ磷酸酶处理组, 所用抗体为 Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb 兔单抗(上)和GSK-3α/β (D75D3) XP ® Rabbit mAb 兔单抗#5676 (下)。 |
