Phospho-HER2/ErbB2 (Tyr1196) (D66B7) Rabbit mAb
货号：
6942S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 6942S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-HER2/ErbB2 (Tyr1196) D66B7) Rabbit mAb recognizes endogenous levels of HER2/ErbB2 protein only when phosphorylated at Tyr1196. The antibody may detect other activated ErbB family members. |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
参考图片Western blot analysis of extracts from T47D and MCF7 cells, untreated or treated with human neuregulin-1 #5218 (100 ng/ml, 5 min), using Phospho-HER2/ErbB2 (Tyr1196) (D66B7) Rabbit mAb (upper) and HER2/ErbB2 (29D8) Rabbit mAb #2165 (lower).Western blot法检测下列细胞提取物：未处理的或用human neuregulin-1 #5218 (100 ng/ml, 5 min)处理的T47D 和MCF7细胞。使用抗体为：Phospho-HER2/ErbB2 (Tyr1196) (D66B7) Rabbit mAb (上) 和 HER2/ErbB2 (29D8) Rabbit mAb #2165 (下)。 |
