| 货号 | 2842S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb detects endogenous levels of HER3/ErbB3 proteins only when phosphorylated at Tyr1289. The antibody does not cross-react with other phosphorylated receptor tyrosine kinases at significant levels. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:1600) |
| 供应商 | CST |
| 背景 | HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but it lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins, and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3K (4). Investigators have found that ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). Research studies have demonstrated that in tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, investigators view inhibiting interaction between ErbB3 and ErbB tyrosine kinases as a novel strategy for anti-tumor therapy. |
| 存放说明 | -20C |
| 计算分子量 | 185 |
Western blot analysis of extracts from T-47D cells (serum starved overnight), untreated (-) or treated with Human Neuregulin-1 (hNRG-1) #5218 (100 ng/ml, 15 min; +), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (upper) or HER3/ErbB3 (1B2E) Rabbit mAb #4754 (lower).Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb. Western blot方法检测T-47D细胞（血清饥饿过夜）提取物，细胞未处理（-）或用人神经调节蛋白-1(hNRG-1) #5218(100 ng/ml, 15 min; +)处理，使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (上图) 或HER3/ErbB3 (1B2E) Rabbit mAb #4754 (下图). | |
Immunohistochemical analysis of paraffin-embedded MCF7 cell pellets, untreated (left) or treated with hNRG-1 #5218 (right), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的MCF7细胞球，细胞未处理（左图）或用hNRG-1 #5218处理（右图），使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded HCC827 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的HCC827移植物，HCC827未处理（左图）或λ-磷酸酶处理（右图），使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织，使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded KYSE-450 cell pellets, untreated (left) or treated with Human Epidermal Growth Factor (hEGF) #8916 (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 (upper) or Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (lower). Note lack of reactivity with Phospho-EGFR.免疫组织化学方法检测石蜡包埋的KYSE-450细胞球，细胞未处理（左图）或用人表皮生长因子(hEGF) #8916处理（右图），使用的抗体为Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 (上图) 或Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (下图). 注意缺乏与磷酸化EGFR的反应。 | |
Immunohistochemical analysis of paraffin-embedded HCC827 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的HCC827移植物，HCC827未处理（左图）或λ-磷酸酶处理（右图），使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb. |
