| 货号 | 14525S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but it lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins, and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3K (4). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
Western blot analysis of extracts from T-47D cells, untreated (-) or treated (+) with Human Neuregulin-1 (hNRG-1) #5218 (100 ng/ml, 5 min), using Phospho-HER3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb (upper) and HER3/ErbB3 (1B2E) Rabbit mAb #4754 (lower). | |
Immunoprecipitation of phospho-Her3/ErbB3 (Tyr1328) from T-47D cell extracts treated with Human Neuregulin-1 (hNRG-1) #5218 (100 ng/ml, 5 min) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Her3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Her3/ErbB3 (Tyr1328) (E1J1T) Rabbit mAb. |
