Phospho-IKKε (Ser172) (D1B7) Rabbit mAb
货号：
8766S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 8766S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-IKKε (Ser172) (D1B7) Rabbit mAb recognizes endogenous levels of IKKε protein only when phosphorylated at Ser172. |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
参考图片Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml, indicated times), using Phospho-IKKε (Ser172) (D1B7) Rabbit mAb (upper) or IKKε (D20G4) Rabbit mAb #2905 (lower).Western blot分析THP-1细胞的细胞提取物，通过TPA #4174 (80 nM, 过夜)分离，紧接着使用LPS (1 μg/ml,指示时间)处理，使用抗体是Phospho-IKKε (Ser172) (D1B7) Rabbit mAb (上图) 或 IKKε (D20G4) Rabbit mAb #2905 (下图)。 |
