Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb
货号：
3026S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3026S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Insulin Receptor (Tyr1345) (14A4) Rabbit mAb detects transfected levels of insulin receptor β only when phosphorylated at Tyr1345. The antibody slightly cross-reacts with activated IGF-I receptors and some other activated tyrosine kinases. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).I型胰岛素样生长因子受体（IGF-I）是一种跨膜受体酪氨酸激酶，广泛表达于许多细胞系，并在胎儿和产后组织细胞中表达（1-3）。受体与IGF-I和IGF-Ⅱ配体结合后发生自身磷酸化。激酶结构域的三个酪氨酸残基（Tyr1131，Tyr1135，Tyr1136）是最早确定的三个主要磷酸化位点（4）。三个酪氨酸残基的磷酸化对激酶的活化是必要的（5,6）。胰岛素受体（IR）与IGF-I受体有显著的结构和功能的相似性，都有激酶结构域激活环中对应的酪氨酸残基（Tyr1146/1150/1151）的存在。IR的酪氨酸磷酸化是胰岛素刺激最早的细胞反应之一（7）。自身磷酸化从Tyr1146和Tyr1150或Tyr1151的磷酸化开始，但完全的激酶活性需要三个酪氨酸残基的磷酸化（8）。 |
| 存放说明 | -20C |
| 计算分子量 | 95 |
参考图片Western blot analysis of CHO IR/IRS-1 cells overexpressing human insulin receptor, untreated or treated with insulin (100 nM for 5 min), using Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb (upper) or Insulin Receptor β (4B8) Rabbit mAb #3025 (lower). 对过表达人胰岛素受体的IR/IRS-1 细胞（未处理，或100nM胰岛素处理5分钟），使用Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb兔单抗（上图）或Insulin Receptor β (4B8) Rabbit mAb兔单抗 #3025（下图）进行Western blot分析。 |
