| 货号 | 3023S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Insulin Receptor β (Tyr1361) (84B2) Rabbit mAb detects transfected levels of insulin receptor β only when phosphorylated at Tyr1361. It slightly cross-reacts with activated IGF-I receptor, but does not cross-react with other activated tyrosine kinases. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Western blot analysis of CHO cells overexpressing human insulin receptors untreated or treated with insulin (100 nM for 5 min), using Phospho-Insulin Receptor β (Tyr1361) (84B2) Rabbit mAb (upper) and Insulin Receptor β (4B8) Rabbit mAb #3025(lower). 对过表达人胰岛素受体的CHO细胞（未处理，或100nM胰岛素处理5分钟），使用Phospho-Insulin Receptor β (Tyr1361) (84B2) Rabbit mAb兔单抗（上图）或Insulin Receptor β (4B8) Rabbit mAb 兔单抗#3025（下图）进行Western blot分析。 | |
Cross-reactivity testing of Phospho-Insulin Receptor (Tyr1361) (84B2) Rabbit mAb with activated IGF-I receptors. Various amounts of activated recombinant IGF-I receptor cytoplasmic domain proteins were applied for Western blot analysis, using Phospho-Insulin Receptor (Tyr1361) (84B2) Rabbit mAb (upper) and Phospho-IGF-I Receptor (Tyr1315/1316) (19H7) Rabbit mAb #3024 (lower) respectively. The result showed that Phospho-Insulin Receptor (Tyr1361) (84B2) Rabbit mAb barely cross-reacts with activated IGF-I receptors. Phospho-Insulin Receptor (Tyr1361) (84B2) Rabbit mAb兔单抗与激活的IGF-I受体进行交叉反应测试。多种激活的重组IGF-I受体胞质结构域，分别使用Phospho-Insulin Receptor (Tyr1361) (84B2) Rabbit mAb兔单抗 (上图)和Phospho-IGF-I Receptor (Tyr1315/1316) (19H7) Rabbit mAb兔单抗 #3024 (下图)进行Western blot。结果显示Phospho-Insulin Receptor (Tyr1361) (84B2) Rabbit mAb兔单抗与仅与激活的IGF-I受体有交叉反应。 |
