| 货号 | 8548S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb detects endogenous levels of IP3 receptor only when phosphorylated at Ser1756. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family and is located in the endoplasmic reticulum. IP3R functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3R1, 2, and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual subunits homo- or hetero-oligomerize to form the receptors functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may be a mode of regulation for Ca2+ release (3,4). IP3R1-mediated Ca2+ release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5). |
| 存放说明 | -20C |
| 计算分子量 | 320 |
Western blot analysis of extracts from mouse and rat brain using Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, with IP3 receptor (Ser1756) phosphopeptide, or with IP3 receptor (Ser1756) non-phosphopeptide prior to incubating the membrane. Western blot方法检测小鼠和大鼠脑组织提取物，使用的抗体为Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb. 在抗体孵育之前，通过用不含肽段，或含IP3受体（Ser1756）磷酸肽或IP3（Ser1756）非磷酸化肽段进行预孵育，对该抗体的磷酸化特异性进行验证。 |
