| 货号 | 5610S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb recognizes endogenous levels of IRS-1 protein only when phosphorylated at Ser318. Note: Ser318 is the mouse residue; the corresponding human residue is Ser323. The antibody cross reacts with a inducible nonspecific band at around 57 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
Western blot analysis of extracts from serum-starved C2C12 cells, untreated or insulin-treated (150 nM for 5 min.), using Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb (upper), or IRS-1 Antibody #3407 (lower). Wetern blot检测血清饥饿的C2C12细胞提取物，未处理或用胰岛素（150 nM,5min）处理，所用抗体为 Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb兔单抗(上)和IRS-1 Antibody #3407 (下)。 |
