Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb
货号：
3193S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3193S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb detects endogenous IRS-1 only when phosphorylated at serine 612. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
参考图片Western blot analysis of extracts from differentiated C2C12 and CHO-IR/IRS-1 (CHO transfected with IR and IRS-1 cDNA) untreated or treated with insulin and λ phosphatase, using Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb (upper), or IRS-1 Antibody #2382 (lower). 对分化的C2C12和CHO-IR/IRS-1细胞（用IR-IRS-1 cDNA转染CHO）（未处理，或胰岛素和λ磷酸酶处理），使用Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb鼠单抗（上图）或IRS-1 Antibody #2382（下图）进行Western blot分析。 |
