| 货号 | 3007S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
Western blot analysis of extracts from untreated or UV+TPA-treated HeLa and COS cells, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).Western blot方法检测细胞提取物：未经处理的和UV+TPA处理的HeLa、COS细胞，使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (上图)和MAPKAPK-2 Antibody #3042 (下图)。 | |
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb.Flow cytometric方法检测细胞提取物：未处理（蓝色）和紫外照射（绿色）的HeLa细胞，使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left), UV-treated (center) or UV and lambda phosphatase-treated (right), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测：未经处理的（左侧）、紫外照射的（中间）和UV＋lambda 磷酸酶处理的（右侧）HeLa 细胞。使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (绿色)，肌动蛋白纤维素已被DY-554 phalloidin (红色)标记。 |
