| 货号 | 11992S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb recognizes endogenous levels of MARCKS protein when phosphorylated at Ser159 and Ser163 (human residues). |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5). |
| 存放说明 | -20C |
| 计算分子量 | 75 (rodent), 80 (human) |
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, serum starved overnight and either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb. Western blot方法检测HeLa, NIH/3T3和 C6细胞提取物，细胞血清饥饿过夜后，DMSO处理或TPA #4174 (200 nM, 15 min)处理，使用的抗体为Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb. | |
Immunoprecipitation of phospho-MARCKS (Ser159/163) from HeLa cells, either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb (lanes 3 and 4) or MARCKS (D88D11) XP® Rabbit mAb #5607 (lanes 5 and 6). Lanes 1 and 2 represent 10% of input lysate for each treatment. Western blot analysis was performed using MARCKS (D88D11) XP® Rabbit mAb #5607. Note: MARCKS (D88D11) XP® Rabbit mAb #5607 does not immunoprecipitate phospho-MARCKS (Ser159/163) and was used as a negative control. 免疫沉淀法检测HeLa细胞phospho-MARCKS (Ser159/163)，DMSO处理或TPA #4174 (200 nM, 15 min)处理，使用的抗体为Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb （泳道3和4) 或 MARCKS (D88D11) XP® Rabbit mAb #5607 (泳道5和6).泳道1和2分别代表每种处理方式中加入的10%的裂解物。进行western blot检测，使用的抗体为MARCKS (D88D11) XP® Rabbit mAb #5607.注意：MARCKS (D88D11) XP® Rabbit mAb #5607不能免疫沉淀phospho-MARCKS (Ser159/163)，可作为阴性对照使用。 |
