| 货号 | 12686S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Western blot analysis of HeLa cell extracts, untreated (-) or treated with Nocodazole #2190 (100 ng/ml, 24 hr; +), using Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb (upper), MCM3 (D47B6) Rabbit mAb #4003 (middle) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). 未处理（-）或诺考达唑#2190 (100 ng/ml, 24 hr; +)处理的HeLa细胞提取物，使用Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb（上），MCM3 (D47B6) Rabbit mAb #4003 (中) 和 α-Actinin (D6F6) XP® Rabbit mAb #6487 (下)进行western blot分析。 | |
Immunoprecipitation of Phospho-MCM3 (Ser112) from HeLa cell extracts, untreated (-) or treated with Nocodazole #2190 (100 ng/ml, 24 hr; +), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3 and 4) or Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb (lane 5 and 6). Lanes 1 and 2 are 10% input. Western blot analysis was performed using Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb.未处理（-）或诺考达唑#2190 (100 ng/ml, 24 hr; +)处理的HeLa细胞提取物中的Phospho-MCM3 (Ser112)，使用Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 3 和 4) 或 Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb (lane 5 和6)进行免疫沉淀实验。Lanes1和2是10%的上样量。使用Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb进行western blot分析。 |
