| 货号 | 12251S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb detects endogenous levels of M-CSF receptor only when phosphorylated at Tyr699. This antibody may cross-react with other activated tyrosine kinases including PDGF receptors and EGFR. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Western blot analysis of extracts from GDM1 cells, serum-starved overnight and untreated (-) or treated with Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 (100 ng/ml, 10 min; +), using Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb (upper) or M-CSF Receptor Antibody #3152 (lower).Western blot方法检测GDM1细胞提取物，细胞经血清饥饿过夜后，不处理（-）或用人巨噬细胞集落刺激因子(hM-CSF) #8929 (100 ng/ml, 10 min; +)处理，使用的抗体为Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb (上图)或M-CSF Receptor Antibody #3152 (下图). | |
Immunoprecipitation of Phospho-M-CSF receptor from M-CSF stimulated GDM1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb.免疫沉淀方法检测M-CSF刺激的GDM1细胞提取物中的磷酸化M-CSF受体，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb (泳道3)。泳道1是加入10%蛋白。用Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb进行Western blot分析。 |
