| 货号 | 3155S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 目标/特异性 | Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb detects endogenous levels of M-CSF receptor only when phosphorylated at tyrosine 723. The antibody does not cross-react with related active protein tyrosine kinases. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:75) F (1:800) |
| 供应商 | CST |
| 背景 | Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLC-γ 2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb specifically binds to phosphorylated M-CSF receptors, but not other phosphorylated protein tyrosine kinases. Western blot anlysis of extracts from various cell lines expressing different activated protein tyrosine kinases, using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb (upper). The same membrane was stripped and reprobed with Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) (lower).抗磷酸化M-CSF 受体(Tyr723)的兔源单克隆抗体(49C10)能特异结合磷酸化的M-CSF 受体蛋白，但不与其它磷酸化酪氨酸蛋白激酶结合。用抗磷酸化M-CSF 受体(Tyr723)的兔源单克隆抗体(49C10)（上）对表达不同激活酪氨酸蛋白激酶的各种细胞系的提取物进行免疫印迹检测。同一张膜洗脱之后，用抗磷酸化酪氨酸(P-Tyr-100)的单克隆抗体重新检测（下）。 | |
Immunohistochemical analysis of paraffin-embedded FDCP1/fms cells, untreated (left) or mCSF-treated (right), using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb.用抗磷酸化M-CSF 受体(Tyr723)的兔源单克隆抗体(49C10)对石蜡包埋的经过（右）或未经（左）mCSF处理的FDCP1/fms细胞进行免疫组化检测。 | |
Flow cytometric analysis of Ba/F3-CSF-1R cells, untreated (blue) or mCSF-treated (green), using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb compared to a nonspecific negative control antibody (red).用抗磷酸化M-CSF 受体(Tyr723)的兔源单克隆抗体(49C10)和非特异的阴性对照抗体（红）对经过（绿色）或未经（蓝色）mCSF处理的Ba/F3-CSF-1R细胞进行流式细胞术检测。 | |
Western blot analysis of extracts from NKM-1 cells, untreated or treated with a M-CSFR inhibitor, using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb (upper) or M-CSF Receptor Antibody #3152 (lower).用抗磷酸化M-CSF 受体(Tyr723)的兔源单克隆抗体(49C10)（上）或抗M-CSF 受体的抗体#3152（下）对经过或未经M-CSFR抑制剂处理的NKM-1细胞的提取物进行免疫印迹检测。 |
