Phospho-Met (Tyr1003) (13D11) Rabbit mAb
货号：
3135S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3135S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Met (Tyr1003) (13D11) Rabbit mAb detects endogenous levels of Met only when phosphorylated at Tyr1003. This antibody may cross-react with other activated protein tyrosine kinases. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
参考图片Western blot analysis of extracts from A431 cells, untreated or HGF-treated using Phospho-Met (Tyr1003) (13D11) Rabbit mAb (upper and middle) and Met (25H2) Mouse mAb #3127 (lower). The middle blot was treated with CIP phosphatase before antibody probing.用抗体Phospho-Met (Tyr1003) (13D11) Rabbit mAb (上，中)或Met (25H2) Mouse mAb #3127 (下)对未经处理的或HGF处理的A431细胞的提取物进行免疫印迹检测。中，抗体孵育前先用CIP磷酸酶处理。 |
