Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb【科瑞奥博】

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Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb

货号： 12280S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	12280S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IP/IF-IC
使用方法	WB(1:1000) IP (1:100) IF-IC (1:400)

性能
供应商	CST
背景	MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).
存放说明	-20C
计算分子量	38 kDa MKK6, 40 kDa MKK3

参考图片

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (40 mJ/cm2 with 30 min recovery; right), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).未处理（左）或UV-处理40 mJ/cm2 with 30 min recovery;右)的HeLa细胞，使用Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb（绿色）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (upper), MKK3 (D4C3) Rabbit mAb #8535 (middle), or MKK6 (D31D1) Rabbit mAb #8550 (lower).未处理（-）或使用TPA #4174 (200 nM, 15 min; +)处理的HeLa和NIH/3T3细胞提取物，使用Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9)Rabbit mAB（上），MKK3 (D4C3)Rabbit mAb #8535（中）或MKK6 (D31D1)Rabbit mAb #8550（下）进行western blot分析。

Immunoprecipitation of phospho-MKK3 (Ser189)/MKK6 (Ser207) from HeLa cells, untreated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (lanes 3 and 4) or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lanes 5 and 6). Lanes 1 and 2 are 10% input. Western blot analysis was performed usingPhospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.未处理或经过TPA #4174 (200 nM, 15 min)处理的HeLa细胞phospho-MKK3 (Ser189)/MKK6 (Ser207)，使用Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (lanes 3 and 4) 或Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lanes 5和 6)进行免疫沉淀实验。Lane1和2是10%的上样量。使用Phospho-MKK3 (Ser189)/MKK6 (Ser207) 抗体#9231进行western blot分析。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678可以用作二抗。