| 货号 | 20750S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP,IF,F |
| 目标/特异性 | Cleaved-Caspase-9 (Asp315) (D8I9E) Rabbit mAb recognizes endogenous levels of caspase-9 protein only when cleaved at Asp315. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) Immunofluorescence (Immunocytochemistry) (1:800) Flow Cytometry (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
| 参考文献 | 1 . Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 2 . Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 3 . Liu, X. et al. (1996) Cell 86, 147-157. 4 . Li, P. et al. (1997) Cell 91, 479-489. 5 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 6 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 7 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 8 . Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 9 . Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60. 10 . MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479. |
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or treated with Staurosporine #9953 (1 μM, 3 hr; right), using Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa and Jurkat cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +) or Etoposide #2200 (25 μM, overnight; +), using Cleaved-Caspase-9 (Asp315) (D8I9E) Rabbit mAb (upper), Caspase-9 (C9) Mouse mAb #9508 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide #2200 (25 μM, overnight; green), using Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 fragment (Alexa Fluor® 488 conjugate) #4412 was used as a secondary antibody. |
