| 货号 | 2976S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IHC-P/IHC-F |
| 目标/特异性 | Phospho-mTOR (Ser2448) (49F9) Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. |
| 使用方法 | IHC-P(1:100) IHC-F (1:100) |
| 供应商 | CST |
| 背景 | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). |
| 存放说明 | -20C |
| 计算分子量 | 289 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific). 免疫组织化学方法检测石蜡包埋的人乳腺癌组织，使用抗体为 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb兔单抗 (IHC Specific)。 | |
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) in the presence of control peptide (left) or Phospho-mTOR (Ser 2448) Blocking Peptide #1230 (right). 免疫组织化学方法检测石蜡包埋的人膀胱移行上皮癌组织，左图为control peptide处理的，右图为Phospho-mTOR (Ser2448) Blocking Peptide #1230处理的，使用抗体为 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb 兔单抗(IHC Specific) 。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma untreated (left) or lambda phosphatase-treated (right), using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific). |
