Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb【科瑞奥博】

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Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb

货号： 12146S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	12146S
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IP
目标/特异性	Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb recognizes endogenous levels of NEDD4L protein only when phosphorylated at Ser342.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
背景	Neural precursor expressed, developmentally down-regulated protein 4 (NEDD4) was originally identified as a gene that is highly expressed in the early mouse embryonic central nervous system (1). Subsequently, a family of NEDD4-like proteins have been defined that includes seven members in humans (2). NEDD4 and NEDD4-like (NEDD4L) proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4L have been shown to downregulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations (3,4). The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins; ubiquitination of these proteins is mediated by the HECT domain of NEDD4 and results in their internalization and removal from the plasma membrane. Research studies have shown that mutation of the PY motifs in ENaC proteins is associated with Liddles syndrome, an autosomal dominant form of hypertension (5). In addition to targeting sodium channels, NEDD4L has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation (6). Mouse and human NEDD4 are rapidly cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear (7).
存放说明	-20C
计算分子量	110,135

参考图片

Western blot analysis of extracts from SK-MEL-28 cells, untreated (-) or CIP-treated (+), using Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb (upper) or NEDD4L Antibody #4013 (lower). Western blot检测经CIP-处理或未经CIP-处理的SK-MEL-28细胞提取物，采用Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb (upper) 或NEDD4L Antibody #4013 (lower)。

Western blot analysis of extracts from of HeLa, SK-MEL-28, and NIH/3T3 cells using Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb. Western blot检测HeLa, SK-MEL-28和NIH/3T3细胞提取物，采用Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb。