| 货号 | 4806S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb detects endogenous levels of p105NF-κB only when phosphorylated at serine 933. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with TNF-α (#2169, 20 ng/ml) plus Calyculin A (#9902, 50 nM) for 5 minutes, using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb.Western免疫印迹。用Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb 研究未经处理的和经TNF-α (#2169, 20 ng/ml)和Calyculin A (#9902, 50 nM)处理5 min 的HeLa和NIH/3T3 细胞的细胞提取液。 | |
Western blot analysis of extracts from Vero cells, untreated or treated with TNF-α #2169 (20 ng/ml) for the times indicated, using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 (upper) and NF-κB p105/p50 Antibody #3035 (lower).Western免疫印迹。用Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 (上图) 和 NF-κB p105/p50 Antibody #3035 (下图) 研究未经处理的和经TNF-α #2169 (20 ng/ml)处理一定时间的Vero细胞的细胞提取液。 |
