| 货号 | 8753S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Confocal immunofluorescent analysis of A-431 cells, serum-starved (left), treated with hEGF #8916 (100 ng/mL, 15 min; center), or treated with hEGF and λ phosphatase (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).血清饥饿（左），经过hEGF #8916 (100 ng/mL, 15 min;中),或hEGF和λ phosphatase（右）处理的A-431细胞，使用Phospho-p90RSK (Thr359) (D1E9)Rabbit mAb（绿色）进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded A-431 cell pellets, untreated (left) or treated with hEGF #8916 (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb.未处理（左）或经过hEGF #8916（右）处理的石蜡包埋的A-431细胞，使用Phospho-p90RSK (Thr359) (D1E9)Rabbit mAb进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb.对照（左）或λ phosphatase-处理（右）后的石蜡包埋的人卵巢癌组织，使用Phospho-p90RSK (Thr359) (D1E9)Rabbit mAb进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded colon carcinoma using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照肽（左）或抗原特异性肽（右）存在时，使用Phospho-p90RSK (Thr359) (D1E9)Rabbit mAb对石蜡包埋的结肠癌组织进行免疫组化分析。 | |
Western blot analysis of extracts from HeLa, COS-7, and A-431 cells, starved overnight and either untreated (-) or treated (+) with TPA #4174 (200 nM, 15 min) or Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb.血清饥饿过夜然后不处理（-）或经过TPA #4174 (200 nM, 15 min; +)或人表皮生长因子（hEGF）#8916 (100 ng/mL, 15 min)处理的HeLa, COS-7, 和A-431细胞提取物，使用Phospho-p90RSK (Thr359) (D1E9)Rabbit mAb进行western blot分析。 | |
Immunoprecipitation of phospho-p90RSK (Thr359) from extracts of HeLa cells, serum starved overnight and either untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Normal Rabbit IgG #2729 (lanes 5 and 6) or Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (lanes 3 and 4). Lanes 1 and 2 are 10% input. Western blot analysis was performed using RSK1/RSK2/RSK3 Antibody #9347.血清饥饿过夜然后不处理（-）或经过TPA #4174 (200 nM, 15 min; +)处理的HeLa细胞提取物，使用正常Rabbit IgG #2729 (lanes 5 和6)或Phospho-p90RSK (Thr359) (D1E9)Rabbit mAb（lanes3和4）对phospho-p90RSK (Thr359)进行免疫沉淀实验。Lane1和2是10%上样量。RSK1/RSK2/RSK3抗体#9347用于western blot分析。 |
