Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb
货号：
13064S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 13064S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb recognizes endogenous levels of PFKFB2 protein only when phosphorylated at Ser483. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
参考图片Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or treated with insulin (150 nM, 5 min; +) or IGF-1 (100 ng/ml, 5 min; +), using Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb (upper) or PFKFB2 (D7G5R) Rabbit mAb #13045 (lower). The phospho-specificity of Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb is verified by treating the nitrocellulose membranes with calf intestinal alkaline phosphatase (CIP) and λ protein phosphatase (λ PP) following transfer of proteins from the SDS-PAGE gel to the membranes. 未处理(-)或insulin (150 nM, 5 min; +) 或 IGF-1 (100 ng/ml, 5 min; +)处理的血清饥饿状态的HeLa细胞，使用Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb兔单抗 (上)或 PFKFB2 (D7G5R) Rabbit mAb 兔单抗#13045 (下) 进行western blot分析。Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb兔单抗的磷酸特异性通过使用牛小肠碱性磷酸酶(CIP)和λ蛋白磷酸酶(λPP)处理硝酸纤维素膜并将蛋白转移到SDS-PAGE胶得以确认。 |
