| 货号 | 5243S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb detects endogenous levels of cellular proteins containing the Ser-phospho-Thr-Pro motif. This antibody has minor cross-reactivity with phospho-Thr-Pro motifs. |
| 使用方法 | WB(1:1000) IP (1:100) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Polo-like kinases (PLKs) are Ser/Thr protein kinases that play an essential role during the cell cycle. At least four PLKs exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4 (1). PLKs have a highly conserved amino-terminal kinase domain and a relatively divergent carboxy-terminal domain called the Polo-box domain (PBD). Of the four PLKs, PLK1 is the best characterized (2). PLK1 functions as a key regulator of mitotic events by phosphorylating substrate proteins on centrosomes, kinetochores, the mitotic spindle, and the midbody, and is crucial for proper progression through multiple stages of mitosis (2-4). The PBDs of PLK1 function as a phospho-Ser/Thr-binding module, recognizing the optimal recognition sequence motif Ser-(pSer/pThr)-(Pro/X) (5). Binding of phosphopeptides containing the Ser-(pSer/pThr)-(Pro/X) motif by the PBD in PLK1 relieves its inhibitory function on kinase activity (6). Ser-(pSer/pThr)-Pro peptides are phosphorylated by proline-directed kinases such as cyclin dependent kinases (CDKs). These findings imply that priming phosphorylations on substrates or docking proteins by other mitotic kinases such as CDKs may target PLK1 to its substrates and simultaneously activate its kinase activity. Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb is a useful tool to study PLK1 binding proteins and PLK1 substrates. |
| 存放说明 | -20C |
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated (100 ng/ml, 16 hrs), using Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb. 使用Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb对来自未处理或100ng/ml Nocodazole处理16小时的Hela细胞抽提液进行Western Blot分析。 | |
Immunoprecipitation of extracts from HeLa cells, untreated or nocodazole-treated (100 ng/ml, 16 hrs), using Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb (lanes 1 and 2). 10% input is shown in lanes 3 and 4. Western blots were performed using Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb (upper), RIP (D94C12) XP® Rabbit mAb #3493 (upper middle), MEK1/2 Antibody #9122 (lower middle), and NPM Antibody #3542 (lower). 使用Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb（列1和列2）对来自未处理或100ng/ml Nocodazole处理16小时的Hela细胞抽提液进行免疫共沉淀分析。10%输入显示在列3和4。使用Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb（上）、RIP (D94C12) XP庐 Rabbit mAb #3493（上中）、或MEK1/2 Antibody #9122（中下）和NPM Antibody #3542（下）进行Western blot分析。 |
