Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb【科瑞奥博】

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Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb

货号： 2997S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	2997S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IP/IHC-P
目标/特异性	Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:800)

性能
供应商	CST
背景	Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).
存放说明	-20C
计算分子量	40

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

免疫组织化学方法检测石蜡包埋的人乳腺癌组织，对照（左图）和λ磷酸酶处理（右图）；使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

免疫组织化学方法检测石蜡包埋的人乳腺癌组织，对照肽处理（左图）和抗原特异性肽处理（右图）；使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

Immunohistochemical analysis of paraffin-embedded metastatic SKOV-3 tumor in mouse lung using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

免疫组织化学方法检测石蜡包埋的鼠肺转移的SKOV-3肿瘤组织，使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with insulin (100 nM, 5 minutes) or with insulin and λ phosphatase, using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).

Western blot检测分析血清饥饿的Hela细胞提取物。未处理和胰岛素 (100 nM）处理5分钟或胰岛素和 λ磷酸酶处理的；使用抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb 兔单抗(上图)和 PRAS40 Antibody #2610 (下图)。