货号 | 14041S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP |
使用方法 | WB(1:1000) IP (1:50) |
供应商 | CST |
背景 | The transient phosphorylation of Prdx1 at Tyr194 leads to inactivation of Prdx1 that in turn promotes localized hydrogen peroxide accumulation (8). Evidence suggests that the Tyr194 phosphorylation of Prdx1 is prominent at the wound edge during repair of cutaneous injury in mice (8,9). |
存放说明 | -20C |
计算分子量 | 21 |
Western blot analysis of extracts from A-431 cells, untreated or treated with EGF (50 ng/ml, 15 min), λ phosphatase (13333 Unit/ml, overnight) using Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb (upper) and Prdx1 (D5G12) Rabbit mAb #8499 (lower). | |
Immunoprecipitation of Phospho-Prdx1 (Tyr194) protein from A-431 cells treated with EGF (50 ng/ml, 15 min), using Rabbit (DA1E) mAb XP® Isotype control #3900 (lane 2) or Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb. |