| 货号 | 14978S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | DNA damage, if not repaired, can lead to genome instability and tumorigenesis. Eukaryotic cells use multiple (sometimes overlapping) signaling pathways to respond to agents that cause various types of DNA lesions. Downstream molecules in DNA repair pathways converge on the sites of DNA damage, resulting in cell cycle arrest and repair or apoptosis (1). Rad18 is an E3 ubiquitin ligase recruited to sites of DNA damage. Along with the E2 ubiquitin ligase Rad6, Rad18 is responsible for monoubiquitination of DNA damage proteins including the replication clamp PCNA and the Fanconi anemia core protein FANCD2. Monoubiquitination of these proteins signals to downstream effector molecules and results in the repair of either post-replication repair lesions via the translesion synthesis (TLS) pathway or DNA double strand breaks via homologous recombination (2-4). Phospho-proteomic studies indicate that Ser403 of Rad18 may be phosphorylated by ATM/ATR in response to DNA damage-inducing agents (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 80, 90 |
Western blot analysis of extracts from 293 cells, untreated (-) or UV-treated (+), using Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb (upper) and Rad18 (D2B8) XP® Rabbit mAb #9040 (lower). | |
Immunoprecipitation of phospho-Rad18 (Ser403) from UV-treated 293 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb. | |
Western blot analysis of extracts from 293 cells, untreated (-) or UV-treated (+), using Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb. Following transfer, blots were either mock treated (left) or treated with calf intestinal phosphatase (CIP, right). |
