| 货号 | 6981S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombe Rad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).人的检验点蛋白Rad17和其裂变和芽殖酵母的同源基因(裂殖酵母和酿酒酵母中分别为Rad17和Rad24)参与响应DNA损伤或DNA合成破坏的检验点信号激活（1-4）。基因毒性药物处理人体细胞能够诱导ATM/ATR依赖的RAD17的Ser635和Ser645磷酸化。Rad17磷酸化在DNA损伤细胞的检验点信号中是一个重要的早期事件（5-7）。 |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from HeLa cells, untreated or treated with CIP and λ phosphatase, using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb (upper) or Rad17 (D3G6) Rabbit mAb #8561 (lower).Western blot方法检测Hela细胞，分为非处理组和CIP与λ磷酸酶共处理组，使用的抗体为Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb (上图) 或 Rad17 (D3G6) Rabbit mAb #8561 (下图)。 | |
Western blot analysis of extracts from MRC-5 cells, untreated or UV-treated (100 mJ/cm2, 2 hr recovery), using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb.Western blot方法检测MRC-5细胞提取物，分为非处理组和紫外处理组(100 mJ/cm2, 恢复2小时), 使用的抗体为Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb。 | |
Immunoprecipitation of phosphorylated Rad17 from MRC-5 cells, untreated or UV-treated (100 mJ/cm2, 2 hr recovery), using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb. Western blot was performed using the same antibody. Lanes 1 and 3 are 10% input.从MRC-5细胞免疫沉淀的磷酸化Rad17蛋白，分为非处理组和紫外处理组 (100 mJ/cm2, 恢复2小时)，使用的抗体为 Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb。Western blot使用同一抗体，泳道1和3为10%input。 |
