| 货号 | 8147S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).视网膜母细胞瘤肿瘤抑制蛋白Rb通过控制细胞周期的G1期限制点的进程来调控细胞增殖(1)。Rb具有3个功能特异的结合结构域并能够与转录因子家族的关键调节蛋白E2F、c-Abl酪氨酸激酶和具有保守的LXCXE模体蛋白相互作用(2-4)。CDK介导的细胞周期依赖性磷酸化能够抑制Rb靶向结合并允许细胞周期进程(5)。Rb的失活和随后的细胞周期进程似乎需要伴随周期蛋白E-CDK2磷酸化的cyclin D-CDK4/6初始磷酸化(6)。离体已经观察到不同CDK/cyclin复合物的特异性(6-8)，而且周期蛋白D1为第780位丝氨酸在体磷酸化所必需(9)。 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of extracts from WI-38 cells, serum-starved for 3 days (-) or serum-starved for 3 days followed by treatment with 10% serum for 2 days (+), using Phospho-Rb (Ser608) (D10F2) Rabbit mAb.Western blot 方法检测WI-38细胞提取物，分为血清饥饿3天组(-)或血清饥饿3天后10%血清处理2天组（+），使用的抗体为Phospho-Rb (Ser608) (D10F2) Rabbit mAb。 | |
Immunoprecipitation of phospho-Rb (Ser608) from WI-38 cell extracts using Phospho-Rb (Ser608) (D10F2) Rabbit mAb (lane 2). Western blot was performed using the same antibody. Lane 1 is 10% input.使用Phospho-Rb (Ser608) (D10F2) Rabbit mAb从MCF7细胞提取物中免疫沉淀丝氨酸（608位）磷酸化的Rb蛋白（泳道2）。Western blot使用同一抗体。泳道1为10%input。 | |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-Rb (Ser608) (D10F2) Rabbit mAb (upper) or Rb (D20) Rabbit mAb #9313 (lower).Western blot 方法检测MCF7细胞提取物，分为未处理组(-)或小牛肠碱性磷酸酶及λ磷酸酶处理组 (+), 使用的抗体为Phospho-Rb (Ser608) (D10F2) Rabbit mAb (上图) 或Rb (D20) Rabbit mAb #9313(下图). |
