| 货号 | 12005S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb recognizes endogenous levels of REPS1 protein only when phosphorylated at Ser709. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | REPS1 is a RalBP1-associated EH-homology domain containing protein. The sequence of REPS1 has an EH domain, followed by two proline-rich segments, and a C-terminal coiled-coil domain for binding to RalBP1 (1). The EH domain of REPS1 interacts with the NPF motif of Rab11-FIP2, mediates their colocalization to endosome vesicles, and influences EGFR endocytosis (2). The two proline-rich regions of REPS1 are important for binding to the SH3 domain of GRK/GRB2 and further regulate EGFR downstream signaling. The proline-rich regions of REPS1 have also been shown to interact with the SH3 domain of intersectin1 (ITSN1) and contribute to ITSN1/SGIP1/REPS1 complex formation on clathrin-coated pits (3). Three alternatively spliced isoforms of REPS1 have been identified. |
| 存放说明 | -20C |
| 计算分子量 | 125 |
Western blot analysis of extracts from Jurkat cells, untreated (-) or calf intestinal phosphatase (CIP)-treated (+), using Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (upper) or REPS1 (D6F4) Rabbit mAb #6404 (lower).未处理或经过牛小肠碱性磷酸酶（CIP）处理（+）的Jurkat细胞提取物，使用Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (上) 或 REPS1 (D6F4) Rabbit mAb #6404 (下)进行western blot分析。 | |
Immunoprecipitation of phospho-REPS1 (Ser709) from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (lane 3)对Jurkat细胞提取物中的phospho-REPS1 (Ser709)进行免疫沉淀实验。Lane1是10%上样量。使用Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb进行western blot分析。 |
