| 货号 | 4857S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-IC |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 235 and 236. |
| 使用方法 | WB(1:1000) IHC-P (1:75) IF-IC (1:100) IHC-F (1:75) |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) 兔单抗，免疫组化分析人类结肠癌石蜡切片，结果显示磷酸化S6核糖体蛋白 (Ser235/236)蛋白在细胞质定位。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗，免疫组化分析人类肺癌石蜡切片，其分别孵育对照多肽(左图)和Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (右图)。 | |
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗，免疫组化分析LNCaP细胞石蜡切片，切片分为对照组（左图）和rapamycin处理组（右图）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗，免疫组化分析人类乳腺癌组织石蜡切片。 | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100ng/ml, 20 min), using Phospho-S6 Ribosomal protein (Ser235/236) (91B2) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower). 使用Phospho-S6 Ribosomal protein (Ser235/236) (91B2) Rabbit mAb兔单抗 (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 #2217 (下图)，免疫印迹（Western Blot）分析在NIH/3T3细胞系中Phospho-S6 Ribosomal Protein (Ser235/236)和S6 Ribosomal Protein兔单抗 (5G10)蛋白水平，分为对照组和PDGF处理组(100 ng/ml, 20分钟)。 | |
Immunohistochemical analysis of frozen U-87MG xenograft, showing cytoplasmic localization using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb兔单抗，免疫组化分析U-87MG异种移植物的冰冻切片，结果显示磷酸化S6核糖体蛋白 (Ser235/236)在细胞质定位。 | |
Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
