| 货号 | 15159S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | All Species Expected, |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | Propionyl-Lysine (D3A9R) Rabbit mAb recognizes endogenous levels of proteins only when propionylated at a lysine residue. This antibody does not cross-react with other lysine modifications. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Lysine is subject to a wide array of regulatory post-translational modifications due to its positively charged ε-amino group side chain. The most prevalent of these are ubiquitination and acetylation, which are highly conserved among prokaryotes and eukaryotes (1,2). Acyl group transfer from the metabolic intermediates acetyl-, succinyl-, malonyl-, glutaryl-, butyryl-, propionyl-, and crotonyl-CoA all neutralize lysine’s positive charge and confer structural alterations affecting substrate protein function. Lysine acetylation is catalyzed by histone acetyltransferases, HATs, using acetyl-CoA as a cofactor (3,4). Deacylation is mediated by histone deacetylases, HDACs 1-11, and NAD-dependent Sirtuins 1-7. Some sirtuins have little to no deacetylase activity, suggesting that they are better suited for other acyl lysine substrates (5). |
| 存放说明 | -20C |
| 参考文献 | 1 . Liu, Z. et al. (2014) Nucleic Acids Res 42, D531-6. 2 . Lee, S. (2013) Toxicol Res 29, 81-6. 3 . Lin, H. et al. (2012) ACS Chem Biol 7, 947-60. 4 . Zhang, Z. et al. (2011) Nat Chem Biol 7, 58-63. 5 . Du, J. et al. (2011) Science 334, 806-9. 6 . Chen, Y. et al. (2007) Mol Cell Proteomics 6, 812-9. 7 . Cheng, Z. et al. (2009) Mol Cell Proteomics 8, 45-52. 8 . Newman, J.C. et al. (2012) J Biol Chem 287, 42436-43. |
Western blot analysis of extracts from 293T cells untreated (-) or treated with Sodium-Propionate (Na-Prop, 50 mM; 16 hours), using Propionyl-Lysine [Prop-K] (D3A9R) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). |
