Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
货号：
4671S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4671S |
| 反应种属 | Human/Mouse/Rat/Hamster |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). |
| 存放说明 | -20C |
| 计算分子量 | 46, 54 |
参考图片Western blot analysis of extracts from 293 cells and NIH/3T3 cells, untreated or UV-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb (upper) or SAPK/JNK (56G8) Rabbit mAb (lower).western blot方法检测细胞提取物：未经处理和紫外照射的293 、NIH/3T3 细胞。使用的抗体是Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb (上图)和SAPK/JNK (56G8) Rabbit mAb (下图）。 |
Western blot analysis of purified MAPK phospho-proteins or extracts from NIH/3T3 cells treated with UV light and PDGF, using Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb (upper), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (middle), and Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 (lower).western blot方法检测纯化的磷酸化MAPK蛋白和紫外照射、PDGF处理的NIH/3T3细胞提取物。使用的抗体是Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb (上图), Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (中间), 和Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 (下图）。 |
