| 货号 | 5599S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb detects endogeneous levels of SGK1 protein only when phosphorylated at Ser78. This antibody does not detect isoforms SGK2 or SGK3. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
Western blot analysis of extracts from COS cells transfected with SGK1 using Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb, untreated or following antibody pre-incubation with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide that cannot block the signal. Western blot分析人类SGK1 (+)转染的COS细胞提取物，所用抗体为Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb兔单抗，未处理或使用位点特异性磷酸化肽段封闭信号或使用位点特异性的非磷酸化肽段不能封闭信号。 | |
Western blot analysis of extracts from COS cells, untransfected (-) or transfected with human SGK1 (+), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower). Western blot分析COS细胞提取物，未转染组（-）和采用人类SGK1 (+)转染组，所用抗体为Phospho-SGK1 (Ser78) (D36D11) (上) 或 SGK1 Antibody #3272 (下)。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with H2O2 (4 mM for 15 min), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower). Western blot分析Hela细胞提取物，未转染组（-）和H2O2 (4 mM for 15 min)转染组，所用抗体为Phospho-SGK1 (Ser78) (D36D11) (上) 或 SGK1 Antibody #3272 (下)。 |
