Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb
货号：
8849S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 8849S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb recognizes endogenous levels of SHP-1 protein only when phosphorylated at Tyr564. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 68 |
参考图片Western blot analysis of extracts from various cell lines, untreated (-) or treated (+) with λ phosphatase, using Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb (upper) and SHP-1 (C14H6) Rabbit mAb #3759 (lower). The JCaM1.6 cell line is a Lck kinase-deficient derivative of the Jurkat cell line. Note the corresponding reduction in phospho-SHP-1 (Tyr564) protein in JCaM1.6 cells, relative to wild-type (Jurkat) cells.Western免疫印迹。用Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb (上图) 和 SHP-1 (C14H6) Rabbit mAb #3759 (下图) 研究未经处理(-)的和经λ 磷酸酶处理(+)的各种细胞系的细胞提取液。JCaM1.6细胞系是Lck激酶缺失派生的Jurkat细胞系。注意：与野生型（Jurkat）细胞相比，在JCaM1.6细胞中，磷酸化的SHP-1 (Tyr564) 蛋白相应减少。 |
