| 货号 | 14716S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 78, 74 |
Western blot analysis of extracts from serum starved NIH/3T3 cells, untreated (-) or treated with insulin (150 nM, 5 min; +) or Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and Sin1 (D7G1A) Rabbit mAb #12860 (lower). | |
Immunoprecipitation of phospho-Sin1 (Thr86) from serum starved NIH/3T3 cell extracts treated with insulin (150 nM, 5 min) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb. |
