| 货号 | 5753S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb recognizes endogenous levels of Smad1 protein only when phosphorylated at Ser206. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts of HeLa cells, untreated or UV-treated (60 mJ/cm2 for 2 minutes followed by 1.5 hour recovery), using Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb (upper) and Smad1 Antibody #9743 (lower). 未处理或经UV处理(60 mJ/cm2 处理2分钟，然后撤除并回复)的HeLa细胞，使用Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb兔单抗 (上) 和 Smad1 抗体 #9743 (下)对细胞提取物进行western blot实验分析。 | |
Western blot analysis of extracts from HT-1080 cells, untreated or treated with TPA #4174 (200 nM for 30 minutes), using Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb (upper) and Smad1 Antibody #9743 (lower). 未处理或经TPA #4174 (200 nM ，30分钟)处理的HT-1080细胞，使用Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb 兔单抗 (上) 和 Smad1 抗体 #9743 (下)对细胞提取物进行western blot实验分析。 |
