| 货号 | 20607S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP |
| 目标/特异性 | Phospho-LIN28A (Ser200) (D5I6X) recognizes endogenous levels of LIN28A protein only when phosphorylated at Ser200. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
| 参考文献 | 1 . Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25. 2 . Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4. 3 . Cho, W.C. (2007) Mol Cancer 6, 60. 4 . Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8. 5 . West, J.A. et al. (2009) Nature 460, 909-13. 6 . Ong, K.K. et al. (2009) Nat Genet 41, 729-33. 7 . Yu, J. et al. (2007) Science 318, 1917-20. 8 . Van Hoof, D. et al. (2009) Cell Stem Cell 5, 214-26. |
Western blot analysis of extracts from NCCIT cells either untreated (-) or treated with lambda phosphatase (+) using Phospho-LIN28A (Ser200) (D5I6X) Rabbit mAb (upper), or LIN28A (A177) antibody, #3978 (lower). |
