Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb
货号：
4805S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4805S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).染色体结构维持蛋白1(SMC1)是黏连蛋白复合体的一个染色体蛋白成员，该复合体促进姐妹染色单体结合并在DNA修复中起着重要作用(1,2)。作为S期内部DNA损伤检验点的一部分，ATM/NBS1-依赖的SMC1磷酸化发生在第957位和966位丝氨酸以应答离子辐射(IR) (3)。在经历其他形式的DNA损伤包括紫外线和羟基脲处理的细胞中，SMC1的磷酸化是ATM-非依赖的(4)。尽管SMC1的磷酸化是IR诱导的内S期检验点激活所必需的，但精确的机制尚未阐明且有可能涉及影响SMC1-SMC3相互作用的构象变化(3)。 |
| 存放说明 | -20C |
| 计算分子量 | 145 |
参考图片Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody (upper), SMC1 Antibody #4802 (middle), and DNA-PK Antibody #4602 (lower).Western blot方法检测293 和NIH/3T3 细胞提取物，未处理或者紫外处理，使用的抗体为Phospho-SMC1 (Ser957) (5D11G5) Mouse Monoclonal Antibody (上), SMC1 Antibody #4802 (中)和DNA-PK Antibody #4602 (下)。 |
