| 货号 | 12432S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Src (Ser17) (D7F2Q) Rabbit mAb (upper) or Src (32G6) Rabbit mAb #2123 (lower). | |
未处理（-）或使用λ磷酸酶和牛小肠碱性磷酸酶（CIP）处理（+）的293T细胞抽取物，使用Phospho-Src (Ser17) (D7F2Q) Rabbit mAb兔单抗（上）或Src (32G6)Rabbit mAb兔单抗#2123（下）进行western blot分析。 |
