Phospho-Stat3 (Ser727) (6E4) Mouse mAb
货号：
9136S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9136S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Phospho-Stat3 (Ser727) (6E4) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at serine 727. It does not significantly cross-react with the corresponding phosphorylated serines of other Stat proteins. The antibody does not cross-react with nonphosphorylated Stat3 or with Stat3 phosphorylated at other sites. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 86 |
参考图片Western blot analysis of extracts from serum-starved HeLa cells, untreated or IL-6-treated (100 ng/ml), using Phospho-Stat3 (Ser727) (6E4) Mouse mAb.Western免疫印迹。用Phospho-Stat3 (Ser727) (6E4) Mouse mAb研究未经处理的血清饥饿的HeLa细胞和经过IL-6 (100 ng/ml)处理的饥饿的HeLa细胞的细胞提取液。 |
