| 货号 | 4113S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. |
| 使用方法 | WB(1:2000) IP (1:100) IHC-P (1:100) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79, 86 |
Western blot analysis of extracts from HeLa cells, untreated or treated with IFN-α (100 ng/ml) for 5 minutes, using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (left) or Stat3 Antibody #9132 (right).Western免疫印迹。用 Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (左图) 或Stat3 Antibody #9132 (右图).研究未经处理的和经IFN-alpha(100 ng/ml,5 min)处理的 HeLa 细胞的细胞提取液。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.流式细胞仪研究未经处理的Jurkat细胞(蓝色)和经IFN-α处理的Jurkat细胞(绿色)。所用抗体为Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb。 | |
Immunohistochemical anlysis of paraffin-embedded human breast carcinoma using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or IFN-alpha treated (right), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).共聚焦免疫荧光分析未经处理的HeLa(左图)或者经IFN-α 处理的 HeLa (右图)，所用抗体为Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (绿色) 和 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (红色)。 | |
Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right). |
