| 货号 | 9314S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. |
| 使用方法 | WB(1:1000) IHC-P (1:400) F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from K562, untreated or treated with Gleevec for 1 hour, using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (upper) or Stat5 Antibody #9310 (lower).Western免疫印迹。Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (上图) or Stat5 Antibody #9310 (下图)研究未经处理和经过Gleevec处理1小时的K562细胞的细胞提取液。 | |
Flow cytometric analysis of K562 cells, Gleevec-treated (blue) or untreated (green), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.流式细胞仪研究未经处理(绿色)或经过Gleevec处理(蓝色) 的K562 细胞。所用抗体为Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells, IFNα-treated (left) or untreated (right), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red).共聚焦免疫荧光。Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (绿色) and Pan-Keratin (C11) Mouse mAb #4545 (红色)研究IFNα处理(左图) 或者 未经处理 (右图)的HeLa。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded K562 cell pellets control (left) or STI-571-treated (right) using Phospho-Stat5 (Tyr694) Rabbit mAb.免疫组织化学染色分析石蜡包埋 K562 细胞沉淀(左图) 或者STI-571处理的K562 细胞沉淀(右图)。所用抗体为Phospho-Stat5 (Tyr694) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb。 | |
Immunohistochemical analysis using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right). |
