| 货号 | 8888S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Survivin (Thr34) (D2E11) Rabbit mAb recognizes endogenous levels of survivin protein only when phosphorylated at Thr34. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 16-18 |
Western blot analysis of HT-29 and HeLa cells, untreated or synchronized in mitosis by thymidine block (2 mM, 17 hr) followed by nocodozole treatment (100 ng/ml, 24 hr), using Phospho-Survivin (Thr34) (D2E11) Rabbit mAb (top), Survivin (71G4B7) Rabbit mAb #2808 (middle), or α-Tubulin (11H10) Rabbit mAb #2125 (lower). 免疫印迹分析HT-29和HeLa细胞，未处理或胸腺嘧啶 block (2 mM, 17 hr)同步处理有丝分裂，随后用nocodozole处理(100 ng/ml, 24 hr)，使用抗体为Phospho-Survivin (Thr34) (D2E11) Rabbit mAb 兔单抗（上），Survivin (71G4B7) Rabbit mAb 兔单抗#2808（中）或α-Tubulin (11H10) Rabbit mAb 兔单抗#2125（下）。 |
