Phospho-TAB2 (Ser372) (D5A4) Rabbit mAb
货号：
8155S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 8155S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | TAK1 is a mitogen-activated protein kinase kinase kinase activated by TGF-β and various pro-inflammatory signals (1,2). In vivo, TAK1 activation requires its association with TAK1 binding protein 1 (TAB1), which triggers TAK1 autophosphorylation at Thr184 and Thr187 (3,4). The TAB2 adaptor protein links TAK1 with TRAF6 to mediate TAK1 activation following IL-1 stimulation (5). Once activated, TAK1 phosphorylates the MAPK kinases MKK4 and MKK3/6, which activate JNK and p38 MAPK, respectively. TAK1 and TRAF6 also activate the NF-κB pathway by phosphorylating the NF-κB inducing kinase (NIK) to trigger subsequent activation of IKK (2,6). In addition to TAK1, TAB1 interacts with and activates p38α MAPK (7). Targeted disruption of the TAB1 gene in mice causes a drastic reduction in TAK1 activity and leads to embryonic lethality (8). |
| 存放说明 | -20C |
| 计算分子量 | 80-84 |
参考图片Western blot analysis of extracts from 293T cells, untreated or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml) and Calyculin A (Serine/Threonine Phosphatase Inhibitor) #9902 (CalA) (100 nM, 20 min), using Phospho-TAB2 (Ser372) (D5A4) Rabbit mAb (upper) or total TAB2 (C88H10) Rabbit mAb #3745 (lower). Lysates were then treated with a combination of calf intestinal phosphatase (CIP) and λ-phosphatase to show phospho-specificity.western blot方法检测细胞提取物：用和未用人源Interleukin-1β (hIL-1β) #8900 (50 ng/ml) 、Calyculin A (Serine/Threonine Phosphatase Inhibitor) #9902 (CalA) (100 nM, 20 min)处理的293T细胞。使用的抗体是Phospho-TAB2 (Ser372) (D5A4) Rabbit mAb (上图) 和 total TAB2 (C88H10) Rabbit mAb #3745 (下图)。裂解物用牛小肠磷酸酶 (CIP)和λ-磷酸酶的混合物处理，以显示磷酸化特异性。 |
